Table 2.

Equilibrium binding affinity and kinetic parameters of the interaction between CDTa and CDTb

Buffer	Ligand	ka (×105 M−1s−1)	kd (×10−4 s−1)	KD (nM)
	WT CDTb	1.44	41.3	28.6
pH 7.4	SPS	1.16	30.4	26.2
	PSS	1.03	56.7	55.1
	WT CDTb	1.89	6.19	3.27
pH 7.4, Ca2+	SPS	2.76	5.21	1.89
	PSS	2.2	10.8	4.93
	WT CDTb	4.98	7.63	1.53
pH 5.5	SPS	4.41	7.09	1.61
	PSS	5.84	13.2	2.27
	WT CDTb	1.52	4.36	2.86
pH 5.5, Ca2+	SPS	1.21	3.26	2.69
	PSS	1.64	3.76	2.29

All parameters were obtained in the analysis, which were shown in SI Fig. 4.