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. 2022 Oct 17;13:6127. doi: 10.1038/s41467-022-33852-2

Fig. 3. Inhibition of the Arp2/3 complex results in accumulation of large flat CCSs at the expense of other CCS categories.

Fig. 3

a–d Control PtK2 cells cultured in the presence of 0.1% FBS and DMSO exhibit flat, dome-shaped and spherical CCSs, some of which are associated with branched actin networks (arrows). e, f PtK2 cells treated with 200 μM CK-666 in the presence of 0.1% FBS contain very large flat CCSs occasionally associated with small remaining patches of branched actin network (arrow). Scale bars, 200 nm. g Percentage of different CCS shape categories in PtK2 cells in indicated conditions. Hatched colors indicate a fraction of CCSs associated with branched actin networks within the given category. Data for 10% FBS are the same as in Fig. 1. h Projection area of flat CCSs in PtK2 cells in indicated conditions. Error bars, mean ± SD; ****p < 0.0001 (Kruskal-Wallis with posthoc Dunn’s multiple comparison test).