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Key steps of CTB tracer injection surgery
(A) The back of the mouse is exposed. Identify rib 10-12.
(B and C) (B) The injection region is identified (yellow outline), (C) the injection sites are marked.
(D) CTB tracer solution is injected into the muscle.
(E) CTB tracer solution is injected into both sides in a mouse. Scale bars: 2mm.
