Examples of finished quantification
Cell number for the ventral horns of a T7-T10 mouse spinal cord segment was quantified using the Labkit pixel classifier algorithm.
(A and B) (A) Ventral view and (B) side view.
(C) There were 393 cells identified on the left side (cyan) and 386 cells on the right side (green).
(D) Schematic showing oblique trunk muscle motor neurons labeled with CTB.
(E) 3D view of individual neurons in the spinal cord from T5 to L1.
(F) Distribution of CTB+ ventral horn neurons with various somatic volumes from an intact T8-T9 mouse spinal cord segmented. Scale bars: 100 μm (A and B), 200 μm (E) 50 μm (E, cyan box), 15 μm (E, red box).