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. 2022 Oct 17;12:17352. doi: 10.1038/s41598-022-22461-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The BuscoPhylo pipeline steps; (1) In case of genomes as input sequence type, separate Genome sequence files are provided as inputs. (2) BUSCO searches are launched on each genome sequence with defined lineage. (3–5) The shared Busco Single Copy genes (S-BUSCO) are extracted, and individual alignments are performed for each BUSCO family gene before generating the super-matrix alignment. (6) the ML phylogenomic tree is inferred. (7) Finally, the graphical visualisation of the tree is displayed in addition to providing the overall results files.