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. 2022 Oct 17;5:1100. doi: 10.1038/s42003-022-04040-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a “Top view” of the NE (Nup96, green; Nup62, red; lamin B1, blue). Green circles with a diameter of 150 nm represent the positions of the Nup96 ring. Red circles have a diameter of 50 nm and represent the positions of Nup62. White rectangle indicates the zoomed-in region. b “Side views” of the NE. The numbered rectangles in the general view represent the zoomed-in regions; n, nucleus; c, cytoplasm. c Sum of aligned images of individual NPCs. d Radial distribution of localizations of each protein obtained by averaging 99 NPC particles. e Averaged “side view” profiles of Nup62, Nup96 and lamin B1 obtained by alignment of 66 individual NPCs. Scale bars, 200 nm (a, left), 1 µm (a, right and b, right), 100 nm (b, left), 50 nm (c).