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. 2022 Oct 17;13:6115. doi: 10.1038/s41467-022-33872-y

Fig. 4. Detection of metabolic intermediate in propane oxidation and phylogenetic affiliation of AssA amino acid sequences in ‘Ca. A. nitratireducens’ genome.

Fig. 4

a Partial ion chromatograms (ion transition, m/z: 289 > 147.2) of the enriched culture extracts (n = 3, taken at various time points) revealed a peak at retention time 10.832 min, matching the peak of the iso-propylsuccinate standard. b A peak at retention time 11.078 min (ion transition, m/z: 289 > 147.1), corresponding to the peak from the propylsuccinate standard, was observed for cell extracts from the enriched culture (n = 3 at different sampling points). c Phylogenetic relationship of ‘Ca. A. nitratireducens’ AssA (Red) to other AssA and BssA in the NCBI database. Three AssA subunits (AssA123) found in ‘Ca. A. nitratireducens’ MAG formed a separate cluster. Bootstrap values >90% are shown as black dots on branch nodes. Scale bar represents amino acid substitutions per site.