TABLE 1.
Protein dephosphorylation events with roles in post-mitotic nuclear reassembly. The list contains a selection of known or probable substrates whose dephosphorylation participates in nuclear reassembly events. Elements that are less certain in view of the current literature are followed by a question mark. ND: Not determined. Multisite: many phosphorylation sites were identified and may contribute to the regulation.
| Substrate | Kinase(s) | Site(s) | Phosphatase(s) | Proposed function dephosphorylation | Selected reference(s) |
|---|---|---|---|---|---|
| Joining chromosomes | |||||
| BAF | VRKs | Thr2? Thr3 Ser4 | PP2A PP4 | Enhances DNA binding | Nichols et al. (2006), Asencio et al. (2012) |
| Zhuang et al. (2014) | |||||
| Mehsen et al. (2018) | |||||
| Torras-Llort et al. (2020) | |||||
| Ki-67 | CDK1 | Multisite | PP1? | Promotes chromosome clustering | Booth et al. (2014) |
| Yamazaki et al. (2022) | |||||
| Membrane recruitment | |||||
| LBR | SRPK1 CDK1 | RS motifs Ser71, Ser86 | PP1 PP2A? | Promotes LBR multimers, Enhances chromatin binding | Takano et al. (2004), Tseng and Chen (2011) |
| Mimura et al. (2016) | |||||
| Emerin | ND | Multisite? | ND | Promotes binding to BAF | Hirano et al. (2005) |
| Man1 | ND | Multisite? | ND | Promotes binding to BAF | Hirano et al. (2009) |
| Lamina assembly | |||||
| Lamin B1/B2 | CDK1 PKC | Multisite | PP1-AKAP149? PP2A? | Polymerization of nuclear lamins, lamina reassembly | Peter et al. (1990), Peter et al. (1991) |
| Goss et al. (1994) | |||||
| Thompson et al. (1997) | |||||
| Steen et al. (2000) | |||||
| Kuga et al. (2010) | |||||
| Kauko et al. (2020) | |||||
| Lamin A/C | CDK1 PKC | Ser22 + Multisite | PP1γ-Repo-Man PP2A? | Polymerization of nuclear lamins, lamina reassembly | Heald and McKeon (1990) |
| Eggert et al. (1993) | |||||
| Kauko et al. (2020) | |||||
| Moriuchi and Hirose (2021) | |||||
| NPC assembly | |||||
| Nup53 | CDK1PLK1 | Multisite | ND | Promotes interactions with multiple Nups within NPC | McCloy et al. (2015), Linder et al. (2017) |
| Martino et al. (2017) | |||||
| Nup98 | CDK1 PLK1NEKs | Multisite | ND | Promotes interactions with multiple Nups within NPC | Laurell et al. (2011) |
| Linder et al. (2017) | |||||
| Nup107 | CDK1 | Multisite | PP2A-B55 | Promotes interaction with other Nups? | McCloy et al. (2015) |
| Cundell et al. (2016) | |||||
| Nup153 | ND | Ser257 | PP2A-B55 PP1γ-Repo-Man? | Promotes recruitment to NE/chromatin in telophase | Cundell et al. (2016) |
| NCD1 | ND | Thr414 | PP2A-B55 | Promotes interaction with other Nups? | Cundell et al. (2016) |
| NE sealing | |||||
| CHMP7 | CDK1 | Ser3 Ser441 | PP1? | Restores interaction with LEM2 for ESCRT-III recruitment to membranes | Asencio et al. (2012) Gatta et al. (2021) |
| LEM2 | ND | Multisite? (disordered region) | PP1? | Restores ability to interact with MTs and oligomerize | Asencio et al. (2012) |
| von Appen et al. (2020) | |||||
| Chromosome decondensation | |||||
| Histone H3 | Haspin Aurora B | Thr3 Ser10 | PP1γ-Repo-Man | Promotes nucleosome acetylation and Interactions | Qian et al. (2011) |
| CAP-H (Condensin I) | Aurora B | Ser70 | ND | Promotes dissociation from chromosomes? | Tada et al. (2011) |
| CAP-G (Condensin I) | CDK1 | Thr308 Thr332 | ND | Promotes dissociation from chromosomes? | Murphy and Sarge (2008) |
| CAP-H2 (Condensin II) | PLK1 | Ser288 | ND | Promotes dissociation from chromosomes? | Kagami et al. (2017) |
| CAP-D3 (Condensin II) | CDK1 | Thr1415 | ND | Prevents PLK1 binding and its phosphorylation of Condensin II? | Abe et al. (2011) |
| Chromatin & Nucleolus organization | |||||
| Histone H3 | Aurora B | Ser10 | PP1γ-Repo-Man | Promotes binding to HP1 | Hirota et al. (2005), Vagnarelli et al. (2011) |
| Nucleophosmin (B23) | CKII CDK1 | Ser125 Thr199 + Multisite | PP1-Ki-67? PP1β,γ-RRP1B? | Promotes nucleolar reassembly? | Okuwaki et al. (2002) |
| Negi and Olsen (2006) | |||||
| Chamousset et al. (2010) | |||||
| Booth et al. (2014) | |||||
| Yamazaki et al. (2022) | |||||
| Kinetochore disassembly | |||||
| Dsn1 | Aurora B | Ser100 Ser109 | PP1-Sds22? PP1-Repo-Man? | Promotes dissociation from CENP-C? | Welburn et al. (2010), Yang et al. (2008) |
| Wurzenberger et al. (2012) | |||||
| Kim and Yu (2015) | |||||
| Rago et al. (2015) | |||||
| CENP-C | CDK1 | Thr734 | ND | Promotes dissociation from CENP-A? | Watanabe et al. (2019) |
| CENP-T | CDK1 | Thr11 Thr85 | ND | Promotes dissociation from Ndc80 complex? | Nishino et al. (2013) |
| Rago et al. (2015) | |||||
| Ser201 | ND | Promotes dissociation from Mis12 complex? | Huis In 't Veld et al. (2016) | ||