. 1999 Sep;6(5):760–764. doi: 10.1128/cdli.6.5.760-764.1999

TABLE 1.

Bacterial strains used in the PCR assay^a

Bacterial strain	Description	Source	PCR template
B. abortus RB51	Rough, derived from 2308 (25)	VPI, Blacksburg, Va.	Bact,^b DNA
B. abortus 2308	Smooth, virulent	VPI, Blacksburg, Va.	Bact, DNA^b
B. abortus 19	Smooth, attenuated	VPI, Blacksburg, Va.	Bact,^b DNA
B. abortus 45/20	Intermediate^c	VPI, Blacksburg, Va.	Bact
B. abortus field strains
Biovar 2	Smooth	NADC, Ames, Iowa	DNA
Biovar 3	Smooth	NADC, Ames, Iowa	DNA
Biovar 4	Smooth	NADC, Ames, Iowa	DNA
Biovar 5	Smooth	NADC, Ames, Iowa	DNA
B. melitensis 16M	Smooth	VPI, Blacksburg, Va.	Bact, DNA^b
B. melitensis Rev1	Smooth	VPI, Blacksburg, Va.	Bact,^b DNA
B. melitensis B115	Rough, O antigen in cytoplasm (11)	VPI, Blacksburg, Va.	Bact,^b DNA
B. melitensis biovar 3	Smooth	NADC, Ames, Iowa	DNA
B. suis biovar 2	Smooth	NADC, Ames, Iowa	DNA
B. suis biovar 3	Smooth	NADC, Ames, Iowa	DNA
B. suis biovar 4	Smooth	NADC, Ames, Iowa	DNA
B. canis RM6/66	Rough	VPI, Blacksburg, Va.	Bact
B. ovis	Rough	VPI, Blacksburg, Va.	Bact
Brucella neotomae	Smooth	NADC, Ames, Iowa	DNA
Ochrobactrum anthropi strains
49237		ATCC, Manassas, Va.	Bact, DNA
49188		ATCC, Manassas, Va.	Bact, DNA
Yersinia enterocolitica O:9		VPI, Blacksburg, Va.	Bact

Abbreviations: Bact, bacteria; VPI, Virginia Polytechnic Institute and State University; NADC, National Animal Disease Center; ATCC, American Type Culture Collection.

Templates used for the PCR amplifications are shown in Fig. 3 and 4.

Strain 45/20 is referred to as intermediate since this strain expresses variable amounts of O side chain (24) and can convert to the smooth phenotype (10).