Individual features per object. The X-axis shows the 5 WSIs and the corresponding feature values are shown on the Y-Axis, with green representing healthy and red, UUO. Examples are taken of the tubule surface area as morphological feature (a), number of nuclei in the interstitium (b), unstained area in glomeruli as color feature (c), mean PAS intensity in the glomeruli (d), glomeruli circularity as morphology feature (e), area occupied by light magenta color in BMC as color feature (f), nuclei density within BMC (g), mean PAS intensity in the BMC (h), and the percentage of dark PAS area in tubule as color feature (i). Note that while all other examples show statistically significant differences (p ≤ 0.005), (c) is an example where no consistent significant differences (p ≤ 0.005) were found and was ultimately rejected in the feature selection step. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)