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IFA testing of human sera for reactivity against N. caninum. Slides were prepared with N. caninum tachyzoites as described in the text and treated with a 1:100 dilution of human sera. (A) Positive reactivity showing uniform peripheral staining of tachyzoites; (B) positive reactivity showing enhanced apical staining in addition to uniform peripheral staining of tachyzoites; (C) negative reactivity showing strong apical staining but no peripheral staining of tachyzoites.
