Table 2.

Mean MCC and HD scores on HCT and HAC lymph node segmentation methods without and with normalization using Macenko, Vahadane, and Reinhard methods. In case of normalization methods, the mean MCC and HD scores of the test scanners are upon normalizing with best performing scanners (e.g. Scanner B and A in HCT and HAC, respectively). P-values are based on MCC scores from all scanners.

Methods		Test scanner	MCC	HD (μm)	P-value
No normalization	HCT	A	0.80 ± 0.21	185.43 ± 126.12	6.09E − 08
		B	0.81 ± 0.26	123.52 ± 54.36
		C	0.80 ± 0.25	214.94 ± 184.80
		D	0.76 ± 0.29	185.48 ± 91.44
	HAC	A	0.87 ± 0.21	165.76 ± 98.53	5.78E − 08
		B	0.82 ± 0.27	120.58 ± 49.43
		C	0.85 ± 0.26	171.63 ± 96.46
		D	0.81 ± 0.29	177.58 ± 82.63
Macenko	HCT	A	0.77 ± 0.24	189.20 ± 106.38	2.29E − 11
		B	0.81 ± 0.26	123.52 ± 54.36
		C	0.79 ± 0.25	175.36 ± 76.02
		D	0.69 ± 0.34	206.14 ± 116.46
	HAC	A	0.87 ± 0.21	165.76 ± 98.53	1.27E − 09
		B	0.79 ± 0.29	124.27 ± 49.31
		C	0.85 ± 0.25	153.31 ± 62.57
		D	0.75 ± 0.35	193.25 ± 107.46
Vahadane	HCT	A	0.67 ± 0.35	243.07 ± 196.36	4.25E − 09
		B	0.81 ± 0.26	123.52 ± 54.36
		C	0.79 ± 0.25	176.36 ± 78.40
		D	0.58 ± 0.43	253.35 ± 171.29
	HAC	A	0.87 ± 0.21	165.76 ± 98.53	3.72E-09
		B	0.77 ± 0.30	126.44 ± 47.76
		C	0.85 ± 0.23	155.72 ± 64.58
		D	0.62 ± 0.43	241.99 ± 158.82
Reinhard	HCT	A	0.74 ± 0.30	222.00 ± 192.50	1.29E − 10
		B	0.81 ± 0.26	123.52 ± 54.36
		C	0.64 ± 0.32	237.24 ± 129.03
		D	0.71 ± 0.31	226.80 ± 166.68
	HAC	A	0.87 ± 0.21	165.76 ± 98.53	1.53E − 05
		B	0.76 ± 0.35	159.10 ± 110.34
		C	0.75 ± 0.35	197.78 ± 103.86
		D	0.76 ± 0.35	212.37 ± 176.00