Table 4.

Mean MCC and HD values using U-Net-based lymph node segmentation by stain color normalizing WSI of test scanner to train scanner (mosaic-based normalization) by using using Macenko, Vahadane, and Reinhard methods. P-values are based on MCC scores between train and normalized scanners.

Methods	Train scanner	Normalized scanner	MCC	HD (μm)	P-value
Macenko	A	A	0.80 ± 0.22	141.95 ± 82.36	0.190
		B	0.74 ± 0.27	84.98 ± 44.72
		C	0.73 ± 0.27	150.04 ± 74.95
		D	0.73 ± 0.29	149.56 ± 80.04
	B	A	0.60 ± 0.39	175.15 ± 102.93	0.002
		B	0.81 ± 0.20	87.52 ± 46.23
		C	0.73 ± 0.27	149.89 ± 66.38
		D	0.65 ± 0.35	161.21 ± 84.03
	C	A	0.52 ± 0.41	176.48 ± 83.22	5.37E − 18
		B	0.76 ± 0.27	86.29 ± 42.75
		C	0.81 ± 0.19	156.70 ± 79.84
		D	0.62 ± 0.37	167.56 ± 90.85
	D	A	0.55 ± 0.30	203.10 ± 119	3.58E − 19
		B	0.78 ± 0.25	87.64 ± 50.55
		C	0.77 ± 0.25	144.48 ± 76.96
		D	0.83 ± 0.19	149.67 ± 83.95
Vahadane	A	A	0.80 ± 0.22	141.95 ± 82.36	1.83E − 04
		B	0.73 ± 0.27	84.86 ± 43.82
		C	0.70 ± 0.28	153.00 ± 75.57
		D	0.58 ± 0.36	177.73 ± 98.97
	B	A	0.46 ± 0.49	190.18 ± 118.82	1.81E − 07
		B	0.81 ± 0.20	87.52 ± 46.23
		C	0.72 ± 0.27	157.17 ± 74.03
		D	0.49 ± 0.49	190.49 ± 106.54
	C	A	0.40 ± 0.52	188.89 ± 99.36	5.61E − 17
		B	0.75 ± 0.28	87.33 ± 44.97
		C	0.81 ± 0.19	156.70 ± 79.84
		D	0.47 ± 0.50	197.69 ± 116.63
	D	A	0.49 ± 0.39	202.32 ± 112.66	1.81E − 23
		B	0.77 ± 0.26	88.21 ± 51.92
		C	0.76 ± 0.26	148.11 ± 82.23
		D	0.83 ± 0.19	149.67 ± 83.95
Reinhard	A	A	0.80 ± 0.22	141.95 ± 82.36	0.44
		B	0.76 ± 0.26	85.08 ± 52.23
		C	0.78 ± 0.23	143.79 ± 73.66
		D	0.77 ± 0.26	145.12 ± 84.41
	B	A	0.74 ± 0.24	140.53 ± 82.76	0.11
		B	0.81 ± 0.20	87.52 ± 46.23
		C	0.76 ± 0.25	139.67 ± 66.05
		D	0.75 ± 0.25	145.39 ± 81.53
	C	A	0.71 ± 0.25	149.52 ± 92.46	1.13E − 05
		B	0.71 ± 0.25	91.69 ± 54.42
		C	0.81 ± 0.19	156.70 ± 79.84
		D	0.72 ± 0.25	156.30 ± 92.03
	D	A	0.76 ± 0.22	149.22 ± 98.30	6.02E − 07
		B	0.75 ± 0.23	93.85 ± 55.99
		C	0.78 ± 0.22	154.53 ± 90.29
		D	0.83 ± 0.19	149.67 ± 83.95