Figure 5.
YY1 is an upstream molecule of FGL1 and participates in the transcriptional regulation of FGL1. (A) Transcription factor of FGL1 was predicted in PROMO and hTFtarget. (B) The level of YY1 mRNA was knocked down by siRNA in PC9 and HCC827 cells (P < 0.0001); the mRNA expression of FGL1 also decreased (P < 0.05). (C) The correlation of YY1 and FGL1 was predicted in GEPIA and validated in tissue ChIP. (D) ChIP-qPCR was performed in PC9 cells to further verify the direct binding of YY1 and FGL1. (E) Dual luciferase reporter assay was performed in HEK 293T cells, which shows once again that YY1 is the transcription factor of FGL1 and has four binding sites. ChIP, chromatin immunoprecipitation. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.