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. 2022 Oct 4;13:1014053. doi: 10.3389/fimmu.2022.1014053

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Copyright © 2022 Tang, Xiong, Zhao, Yang, Shi, Zheng, Liu, Shu, Jiang, Ma and Zhao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MYH9 is a downstream molecule of FGL1. (A) IP was used to adsorb and precipitate related proteins that FGL1 might bind to. (B) MS was performed and the top 10 FGL1-combined proteins were listed—MYH9 was the most likely protein to bind to FGL1. (C) GO analysis of the results of MS with five subclasses, including biological process, cellular component, molecular function, protein class, and pathway analysis. (D) Validation by western blot supports that MYH9 is the main downstream molecule of FGL1. IP, immunoprecipitation; MS, Mass spectrometry. *, P < 0.05; **, P < 0.01; ***, P < 0.001.