Figure 6.

Relatively normalized expression (RNE) of COL10A1 in solid tumor, adjacent normal colon tissue, and CRC and fibroblast cell lines. For RNE, the sample with the lowest CT value is set as reference (value: 1) for other tissues and cell lines. CT values were normalized on GAPDH. (A) Illustration of multiple increased expression levels in CRC tumor stroma compared with in vitro cultured CRC cell lines and fibroblasts. (B) Enlarged breakdown of COL10A1 expression levels of common fibroblast and CRC cell lines. Fibroblasts (CCL186) were shown to have an eightfold increased expression compared to the strongest expressing tumor cell SW620.