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. 2022 Oct 14;222(1):e202112108. doi: 10.1083/jcb.202112108

Figure 1.

Figure 1.

Reduced lifespan of GARP knockout flies. (A) Cartoon depicting the GARP and EARP complexes. (B) RT-PCR from control (+/+) and KOs. RNA was extracted from whole larvae, reverse transcribed, and equal amounts of cDNA were used for RT-PCR. RP49 is used as a reference gene. Bp = base pairs. (C and D) Survival curves and average age at death ± SEM for male flies of the indicated genotypes. N > 200 flies/genotype. Survival curves were analyzed by Log-Rank Mantel-Cox test with Bonferroni multiple comparisons correction. ****P < 0.0001 for all genotypes compared to control except Vps50KO/Df (not significant—n.s.). Average age at death compared to +/+ was analyzed by one-way ANOVA with Dunnett’s post-test. ****P < 0.0001. (E and F) Survival curves and average age at death ± SEM for female flies of the indicated genotypes. N > 188 flies/genotype. Survival curves were analyzed as in C. ****P < 0.0001 for all genotypes compared to control except Vps50KO/Df (n.s.). Average age at death analyzed as in D. ****P < 0.0001. Source data are available for this figure: SourceData F1.