Vps53KO/KO
MARCM clonal neurons have smaller dendritic arbors. (A) Representative maximum z-projections of MARCM control FRT40A, Vps53KO/KO, and Vps53KO/KO; ppk > Vps53 c4da neuron clones labeled with ppk-Gal4, UAS-CD4-tdGFP. Images were collected from 7-d-old male pharate adults. Yellow arrows point to the soma. Scale bar = 50 μm. (B) Quantification of total dendrite branch length, ****P < 0.0001, n.s. P = 0.1797. (C) Quantification of total branch number, ****P < 0.0001, n.s. P = 0.2579. Both total dendrite branch length and number were analyzed by one-way ANOVA with Tukey’s post-test. Data presented as mean ± SD. (D) Sholl analysis. Curves are the average number of intersections/radius. For B–D, MARCM control FRT40A
n = 8 independent neurons; Vps53KO/KO
n = 13 independent neurons; and Vps53KO/KO; ppk > Vps53 n = 11 independent neurons. Flies were collected from at least three independent experiments.