Figure 3.

Both the GARP and EARP complexes are necessary for dendrite arborization in class 4 da neurons. (A) Representative maximum intensity z-projections of class IV da neurons labeled with ppk-Gal4, UAS-CD4-tdTomato from 7-d-old male pharate adults. Yellow arrows point to the soma. Scale bar = 50 μm. Both total dendrite branch length and number were analyzed by one-way ANOVA with Tukey’s post-test. Data presented as mean ± SD. (B) Quantification of total dendrite length ***P = 0.0001, ****P < 0.0001, +/+ vs. Vps50^KO/KO; ppk > Vps50, P = 0.278, +/+ vs. Vps54^KO/KO; ppk > Vps54, P = 0.3165m, Vps50^KO/KO vs. Vps54^KO/KO ***P = 0.0003. (C) Quantification of total dendrite branch number. +/+ vs. Vps54^KO/KO; ppk > Vps54, *P = 0.0107, Vps54^KO/KO vs. Vps54^KO/KO; ppk > Vps54, *P = 0.0178), +/+ vs. Vps50^KO/KO **P = 0.009, ****P < 0.0001, Vps50^KO/KO vs. Vps54^KO/KO ****P < 0.0001, remaining n.s. comparisons P > 0.27. (D) Sholl analysis. Curves are the average number of intersections/radius. For B–D, n = 7–12 independent neurons/genotype. (E) Quantification of total dendrite branch length over development from 72 h APF to 21 d after eclosion. Analyzed by two-way ANOVA with Tukey’s post-test. Data presented as mean ± SD.**P < 0.01, ***P < 0.001, ****P < 0.0001. Please see Fig. S2, D–F for additional statistics and Table S2 for the full list of P values for the comparisons in E. +/+ n = 10 independent neurons/timepoint; Vps50^KO/KO n = 9–11 independent neurons/timepoint; Vps54^KO/KO n = 10–12 independent neurons/timepoint. For B–E, flies were collected from at least three independent experiments.