Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 14;222(1):e202112108. doi: 10.1083/jcb.202112108

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 O'Brien et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Figure 8. — Depletion of Osbp rescues TGN but not late endosomal morphology in GARP KO neurons. (A) Representative maximum intensity z-projections of endogenous Golgin245 staining from c4da neurons in pupae 96 h APF. Dashed line shows soma area. Scale bar = 1 μm. For B–D, n = 22–27 independent samples/genotype. All data in this figure were analyzed by one-way ANOVA with Tukey’s post-test. Samples were obtained from at least three independent experiments. (B) Quantification of the number of Golgin245 puncta/soma. +/+ vs. Vps54^KO/KO ****P < 0.0001, Vps54^KO/KO vs. Vps54^KO/KO; Osbp^1/+ *P = 0.0404. +/+ vs. Vps54^KO/KO; Osbp^1/+ n.s. P = 0.1728. (C) Golgin245 fluorescence intensity. +/+ vs. Osbp^1/+ *P = 0.0406. +/+ vs. Vps54^KO/KO n.s. P = 0.0808, +/+ vs. Vps54^KO/KO; Osbp^1/+ P = 0.0683. (D) Golgin puncta area. n.s. P > 0.24. (E) Representative maximum intensity z-projections of endogenous Rab7 staining from c4da neurons in pupae 96 h APF. (F–H) For F–H, n = 23–28 independent samples/genotype. (F) Quantification of the number of Rab7 puncta in the soma. +/+ vs. Vps54^KO/KO **P = 0.0067. +/+ vs. Osbp^1/+ **P = 0.0013. +/+ vs. Vps54^KO/KO; Osbp^1/+ **P = 0.0029^. Other comparisons n.s. P > 0.98. (G) Rab7 fluorescence intensity. +/+ vs. Osbp^1/+ **P = 0.0404. Other comparisons P > 0.11. (H) Rab7 puncta area. All comparisons, n.s. P > 0.36.