Figure 6.

Colonic motility in rats without or with the administration of a transient receptor potential ankyrin 1 (TRPA1) inhibitor. (A) Decreased ratio of peak frequency (PF) in high-amplitude pressure following the administration of 5°C saline in rats without administration of a TRPA1 inhibitor. To quantify the decrease in high-amplitude pressure induced by 5°C saline, the ratio of contraction frequency before and after administration with a length of more than 8 mm was calculated as the %PF. **P < 0.01 vs room temperature (25°C) without a TRPA1 inhibitor, ***P < 0.001 vs room temperature (25°C) with a TRPA1 inhibitor. (B) The area under the curve (AUC) at high-amplitude pressure with 5°C saline. The AUC was calculated before and after administration of saline using the lowest intraluminal pressure value as the baseline. There were no significant differences between the 2 groups. (C) Results of the peak pressure amplitude (PPA) at high-amplitude pressure with 5°C saline. The difference between the PPA and the lowest intraluminal pressure was defined as the PPA, and the ratio of the PPAs before and after medication was calculated as the %PPA. There were no significant differences between the 2 groups (n = 3).