Table 1. The primer sequences for RT-PCR experiments.
Gene | Primer sequences (5'-3') | |
---|---|---|
Forward | Reverse | |
GAPDH | GGAGCGAGATCCCTCCAAAAT | GGCTGTTGTCATACTTCTCATGG |
ADAMTS6 | CCCTTCAACAACGACATCTGT | CCGTTCAATGCTCACTGATCT |
POU5F1 | GGGAGATTGATAACTGGTGTGTT | GTGTATATCCCAGGGTGATCCTC |
NANOG | TTTGTGGGCCTGAAGAAAACT | AGGGCTGTCCTGAATAAGCAG |
SOX2 | TACAGCATGTCCTACTCGCAG | GAGGAAGAGGTAACCACAGGG |
ABCB1 | GGGATGGTCAGTGTTGATGGA | GCTATCGTGGTGGCAAACAATA |
ABCC1 | TTACTCATTCAGCTCGTCTTGTC | CAGGGATTAGGGTCGTGGAT |
ABCG2 | ACGAACGGATTAACAGGGTCA | CTCCAGACACACCACGGAT |
Vimentin | AGTCCACTGAGTACCGGAGAC | CATTTCACGCATCTGGCGTTC |
CDH1 | CGAGAGCTACACGTTCACGG | GGGTGTCGAGGGAAAAATAGG |
TWIST1 | GTCCGCAGTCTTACGAGGAG | GCTTGAGGGTCTGAATCTTGCT |
SNAlL1 | TCGGAAGCCTAACTACAGCGA | AGATGAGCATTGGCAGCGAG |
ZEB1 | TTACACCTTTGCATACAGAACCC | TTTACGATTACACCCAGACTGC |
RT-PCR, reverse transcription-polymerase chain reaction.