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. 2022 Oct 17;10(10):e004973. doi: 10.1136/jitc-2022-004973

Figure 1.

Figure 1

Laser immunotherapy (LIT) cures B16-F10 tumors and confers long-term memory. (A) Schematic of tumor inoculation, LIT treatment, tissue collection, and tumor rechallenge. (B) Survival of animals inoculated with B16-F10 tumors in different treatment groups (untreated, GC alone, PTT alone, or LIT). (C) Survival of LIT-cured animals rechallenged on the opposite flank, compared with untreated first-time challenged controls. Log-rank (Mantel-Cox) test was used for statistical analysis of the survival data. (D) Flow cytometry analysis of the normalized mean expression of CD28 and CD25 on the surface of CD8+ effector and memory T cells from the spleens of animals in different groups 72 hours after treatment. (E) Flow cytometry analysis of the normalized mean expression of CD69 on the surface of naïve CD4 and CD8 naïve T cells from the spleen of animals 7 days after different treatments. One-way analysis of variance was used for statistical analysis of the mean expression data. P values = *0.5, **0.05, **0.005, ****0.0005. GC, N-dihydrogalactochitosan; PTT, photothermal therapy; SQ, subcutaneous.