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. 2022 Oct 17;10(10):e004973. doi: 10.1136/jitc-2022-004973

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© Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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Isolation and characterization of B16-F10 MHC-I peptides. (A) Schematic of the processes of generating tumor extracts, and isolation and identification of H2-Db and H2-Kb peptides. (B) The length distribution, the common motifs, and the total number of non-redundant peptides identified from either the H2-Db or H2-Kb MHC-I allele. (C) Schematic for selection of the tumor-specific peptides to be screened for T-cell reactivity by ELISpot. LC-MS, liquid chromatography coupled mass spectrometry; MHC, major histocompatibility complex.