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. 2022 Sep 11;21(10):e13708. doi: 10.1111/acel.13708

FIGURE 5.

FIGURE 5

Induction of Ntf3 expression starting at 60 weeks of age results in increased IHC synapse densities and ABR peak 1 amplitudes at 95 weeks of age. (a) Representative confocal images of IHC synapses at the 16 kHz region of a 95‐week‐old Ntf3 stop and a Ntf3 stop :Slc1a3/CreER T cochlea immunolabeled for hair cells (MyoVIIa), pre‐synaptic ribbons (Ctbp2) and post‐synaptic receptor patches (GluR2). (b) At 95 weeks of age, Ntf3 overexpressors (Ntf3 stop :Slc1a3/CreER T ) have 14–17% more IHC synapses than their age‐matched controls (Ntf3 stop ), a density not different than that seen in 10‐week‐old controls. 10‐week‐old Ntf3 stop n = 6 cochleae; 95‐week‐old Ntf3 stop n = 5 cochleae; 95‐week‐old Ntf3 stop :Slc1a3/CreER T n = 5 cochleae. Only one cochlea from each mouse was analyzed. 1‐way ANOVA followed by Tukey's multiple comparisons test was used to evaluate differences among the three groups at an individual cochlear region (frequency, kHz). * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 by one‐way ANOVA. Error bars represent SEM. (c) Ntf3 expression levels, ABR peak 1 amplitudes, and IHC synapses density strongly correlate in 95‐week‐old mice. Correlations were evaluated using Spearman correlation test. p < 0.05 were considered as statistically significant differences