FIGURE 6.

Ntf3 overexpression starting at mid‐life does not alter age‐related OHC loss. (a) Representative confocal images of the 16 kHz region of Ntf3 ^stop and Ntf3 ^stop :Slc1a3/CreER ^T cochleae immunolabeled for hair cells (MyoVIIa) showing mild OHC loss without IHC loss in both groups at 95 weeks of age. (b) Quantitative analysis shows no IHC loss. (c) Both groups show equal degrees of mild OHC loss. Ntf3 ^stop n = 3–6 cochleae; Ntf3 ^stop :Slc1a3/CreER ^T n = 5–6 cochleae. Only one cochlea from each mouse was analyzed. 2‐tailed t‐test was used to evaluate differences between Ntf3 ^stop and Ntf3 ^stop :Slc1a3/CreER ^T groups at an individual cochlear region (frequency, kHz). Non‐significant differences between groups were found when evaluating either ICH or OHC survival. Error bars represent SEM