. 2022 Oct 3;13:994151. doi: 10.3389/fmicb.2022.994151

Table 8.

Differential bacteria (%) identified from piglet gut microbiota among groups¹.

	NC	PC	GOD	P-value
Phyla
Fusobacteriota	0.026 ± 0.023^b	2.379 ± 2.208^a	0.052 ± 0.011^b	0.024
Proteobacteria	0.636 ± 0.135	6.410 ± 3.439	1.694 ± 0.855	0.076
Euryarchaeota	0.046 ± 0.016	0.414 ± 0.250	0.061 ± 0.034	0.097
Classes
Clostridia	16.866 ± 1.483^b	29.031 ± 3.007^a	25.799 ± 3.431^a	0.024
Fusobacteriia	0.026 ± 0.023^b	2.379 ± 2.208^a	0.052 ± 0.011^b	0.024
Gammaproteobacteria	0.632 ± 0.135	6.406 ± 3.439	1.691 ± 0.853	0.076
Methanobacteria	0.046 ± 0.016	0.414 ± 0.250	0.061 ± 0.034	0.097
Orders
Mycoplasmatales	0.362 ± 0.250^a	0.041 ± 0.011^b	0.019 ± 0.012^b	0.009
Fusobacteriales	0.026 ± 0.023^b	2.379 ± 2.208^a	0.052 ± 0.011^b	0.024
Clostridiales	2.764 ± 0.450^b	5.783 ± 1.105^a	5.567 ± 0.790^a	0.043
Enterobacterales	0.139 ± 0.054^b	1.449 ± 0.772^a	0.138 ± 0.037^b	0.044
Actinomycetales	0.001 ± 0.001^b	0.006 ± 0.002^a	0.009 ± 0.003^a	0.045
Methanobacteriales	0.046 ± 0.016	0.414 ± 0.250	0.061 ± 0.034	0.097
Families
Mycoplasmataceae	0.362 ± 0.250^a	0.041 ± 0.011^b	0.019 ± 0.012^b	0.009
Fusobacteriaceae	0.026 ± 0.023^b	2.377 ± 2.206^a	0.052 ± 0.011^b	0.024
Erysipelatoclostridiaceae	0.166 ± 0.022^a	0.233 ± 0.128^a	0.080 ± 0.016^b	0.024
Clostridiaceae	2.764 ± 0.450^b	5.783 ± 1.105^a	5.567 ± 0.790^a	0.043
Enterobacteriaceae	0.137 ± 0.054^b	1.448 ± 0.772^a	0.137 ± 0.036^b	0.044
Actinomycetaceae	0.001 ± 0.001^b	0.006 ± 0.002^a	0.009 ± 0.003^a	0.045
Sutterellaceae	0.013 ± 0.006^a	0.015 ± 0.007^a	0.002 ± 0.001^b	0.046
Lactobacillaceae	40.577 ± 4.368	18.146 ± 3.282	30.295 ± 8.332	0.055
Clostridium methylpentosum	0.006 ± 0.002	0.071 ± 0.026	0.019 ± 0.010	0.070
Eggerthellaceae	0.606 ± 0.235	0.700 ± 0.133	0.397 ± 0.133	0.085
Methanobacteriaceae	0.046 ± 0.016	0.414 ± 0.250	0.061 ± 0.034	0.097
Genera
Mycoplasma	0.362 ± 0.250^a	0.041 ± 0.011^b	0.019 ± 0.012^b	0.009
Erysipelotrichaceae_UCG-009	0.177 ± 0.134^a	0.018 ± 0.008^b	0.011 ± 0.009^b	0.010
Veillonella	0.016 ± 0.011^b	0.599 ± 0.533^a	0.039 ± 0.008^b	0.013
Methanosphaera	0.029 ± 0.011^b	0.232 ± 0.127^a	0.002 ± 0.002^b	0.017
Sutterella	0.005 ± 0.001^a	0.005 ± 0.003^a	0.001 ± 0.001^b	0.022
Solobacterium	0.738 ± 0.174^b	1.282 ± 0.212^a	0.572 ± 0.179^b	0.022
Fusobacterium	0.026 ± 0.023^b	2.377 ± 2.206^a	0.052 ± 0.011^b	0.024
Escherichia/Shigella	0.130 ± 0.054^b	1.398 ± 0.750^a	0.129 ± 0.035^b	0.034
Clostridium_sensu_stricto_1	2.710 ± 0.442^b	5.677 ± 1.100^a	5.343 ± 0.784^a	0.037
Candidatus_Soleaferrea	0.011 ± 0.005^b	0.048 ± 0.013^a	0.033 ± 0.022^a ^b	0.042
Methanobrevibacter	0.017 ± 0.006	0.181 ± 0.128	0.059 ± 0.034	0.050
Senegalimassilia	0.329 ± 0.172	0.392 ± 0.118	0.136 ± 0.062	0.051
Lactobacillus	40.577 ± 4.368	18.146 ± 3.282	30.295 ± 8.332	0.055
Prevotella	13.244 ± 4.002	5.692 ± 1.997	5.805 ± 2.262	0.067
Megasphaera	1.097 ± 0.283	0.275 ± 0.087	2.028 ± 0.702	0.083
Erysipelotrichaceae_UCG-003	0.016 ± 0.005	0.006 ± 0.002	0.004 ± 0.002	0.083
Lachnospiraceae_AC2044	0.022 ± 0.008	0.048 ± 0.012	0.239 ± 0.143	0.088
Ruminococcaceae UCG-005	0.504 ± 0.095	1.505 ± 0.404	2.324 ± 1.013	0.089
Species
Lactobacillus_amylovorus	23.682 ± 3.057^a	5.522 ± 0.912^b	8.974 ± 3.567^b	0.008
Metamycoplasma_sualvi	0.359 ± 0.251^a	0.028 ± 0.012^b	0.019 ± 0.011^b	0.008
Roseburia_hominis	0.060 ± 0.013^a	0.049 ± 0.036^a ^b	0.019 ± 0.004^b	0.009
Veillonella_magna_	0.011 ± 0.008^b	0.418 ± 0.380^a	0.022 ± 0.003^b	0.018
Lactobacillus_salivarius	0.044 ± 0.021^a	0.006 ± 0.003^b	0.076 ± 0.068^a	0.019
Fusobacterium_necrophorum	0.024 ± 0.022^b	2.330 ± 2.164^a	0.051 ± 0.011^b	0.029
Escherichia_coli	0.130 ± 0.054^b	1.397 ± 0.749^a	0.129 ± 0.035^b	0.036
Megasphaera_elsdenii	1.083 ± 0.279	0.271 ± 0.085	2.011 ± 0.698	0.083

^a,b

Values with different superscripts differ significantly (p < 0.05).

NC, negative control (piglets were free of the challenge); PC, positive control (piglets were challenged with ETEC on the 11th day of the experiment); GOD, PC piglets supplemented with 200 g/t glucose oxidase. Samples were analyzed on the 21st day of the experiment.