. 2022 Oct 3;13:994151. doi: 10.3389/fmicb.2022.994151

Table 9.

Comparison of the predicted pathways of piglet gut microbiota among groups¹.

	NC	PC	GOD	P-value
Nutrient metabolism-related pathways
D-Glutamine and D-glutamate metabolism	2.603 ± 0.042^a	2.409 ± 0.129^b	2.514 ± 0.210^a ^b	0.009
Fatty acid degradation	0.331 ± 0.009^b	0.371 ± 0.037^a	0.351 ± 0.027^a ^b	0.019
Fructose and mannose metabolism	1.316 ± 0.065^a	1.105 ± 0.140^b	1.245 ± 0.233^a ^b	0.021
Linoleic acid metabolism	0.225 ± 0.027^b	0.266 ± 0.050^a	0.277 ± 0.032^b	0.022
Purine metabolism	1.076 ± 0.027^a	0.993 ± 0.053^b	1.028 ± 0.082^a ^b	0.022
Aminoacyl-tRNA biosynthesis	2.005 ± 0.042^a	1.875 ± 0.104^b	1.943 ± 0.178^a ^b	0.029
Lysine biosynthesis	1.768 ± 0.071^a	1.642 ± 0.076^b	1.655 ± 0.105^b	0.031
Primary bile acid biosynthesis	0.311 ± 0.078^a	0.176 ± 0.063^b	0.256 ± 0.136^a ^b	0.034
Secondary bile acid biosynthesis	2.799 ± 0.701^a	1.582 ± 0.566^b	2.306 ± 1.225^a ^b	0.034
Pyrimidine metabolism	1.458 ± 0.037^a	1.346 ± 0.093^b	1.412 ± 0.125^a	0.039
Valine, leucine and isoleucine degradation	0.301 ± 0.029^b	0.376 ± 0.063^a	0.344 ± 0.074^a ^b	0.044
Amino sugar and nucleotide sugar metabolism	1.375 ± 0.063^a	1.226 ± 0.112^b	1.316 ± 0.192^a ^b	0.050
Starch and sucrose metabolism	1.181 ± 0.042	1.065 ± 0.108	1.104 ± 0.094	0.058
Lysine degradation	0.133 ± 0.008	0.170 ± 0.039	0.147 ± 0.031	0.069
Taurine and hypotaurine metabolism	0.889 ± 0.078	0.770 ± 0.063	0.838 ± 0.146	0.075
Other glycan degradation	0.960 ± 0.183	0.733 ± 0.248	0.974 ± 0.426	0.088
Sphingolipid metabolism	0.367 ± 0.034	0.293 ± 0.066	0.316 ± 0.135	0.099
Bacterial pathogenicity-related pathways
Bacterial invasion of epithelial cells	0.002 ± 0.001^b	0.007 ± 0.003^a	0.001 ± 0.001^b	0.008
Bacterial chemotaxis	0.591 ± 0.090^b	1.107 ± 0.420^a	0.843 ± 0.433^a ^b	0.015
Flagellar assembly	0.361 ± 0.052^b	0.678 ± 0.264^a	0.537 ± 0.288^a ^b	0.025
Shigellosis	0.001 ± 0.001^b	0.006 ± 0.003^a	0.001 ± 0.001^b	0.034

^a,b

Values with different superscripts differ significantly (p < 0.05).

NC, negative control (piglets were free of the challenge); PC, positive control (piglets were challenged with ETEC on the 11th day of the experiment); and GOD, PC piglets supplemented with 200 g/t glucose oxidase. Samples were analyzed on the 21st day of the experiment.