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(A) Denaturing polyacrylamide gel-analysis of oligonucleotides before (desalted) and after PAGE purification (PAGE-purified). The gels are stained with ethidium bromide. (B) Autoradiogram of annealing reaction products after native PAGE. U: unannealed oligonucleotide; S: Substrate; 1* and 2*: Higher molecular weight products likely corresponding to intermolecular G4s.
