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. Author manuscript; available in PMC: 2023 Mar 25.
Published in final edited form as: Methods Enzymol. 2022 Mar 25;672:233–260. doi: 10.1016/bs.mie.2022.02.020

Figure 5: CMG helicase assay.

Figure 5:

(A) Wildtype (left) or mutant (right) G4 on translocation strand; (B) Wildtype (left) or mutant (right) G4 on non-template strand. (C) Wildtype G4 sequence (left) or random DNA sequence (right) in B-form duplex DNA. Red star indicates the position of radiolabel. Reaction products were separated by native PAGE and imaged on a phosphor screen. Product formation is quantified using ImageJ and plotted using Prism 9 software.