| Component | Volume |
|---|---|
| 10x PNK Buffer | 3 μl |
| ddH2O | 20.25 μl |
| Oligonucleotide A/C (10 μM) | 0.75 μl |
| γ-P32-ATP | 4.5 μl |
| PNK | 1.5 μl |
| Total | 30 μl |
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