| Component | Volume |
|---|---|
| 10x Helicase Buffer | 3.5 μL |
| DTT (100 mM) | 0.9 μL |
| BSA (1 mg/mL) | 3.5 μL |
| ATP (100 mM) | 3.5 μL |
| ddH2O | 23.6 μL |
| Total | 35 ml |
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