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. 2022 Sep 30;10:896297. doi: 10.3389/fcell.2022.896297

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Copyright © 2022 Groenendyk, Stoletov, Paskevicius, Li, Dai, Pujol, Busaan, Ng, Boukouris, Saleme, Haromy, Cui, Hu, Yan, Zhang, Michelakis, Chen, Lewis, Tang, Agellon and Michalak.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Migration of the HT1080tdT-ε2A5 fibrosarcoma cells. (A). An FITC-gelatin degradation assay for invasiveness of HT1080tdT and HT1080tdT-ε2A5 cells. Samples were co-stained with DAPI and actin. *p < 0.0001, n = 3. (B). Transwell migration of HT1080tdT and HT1080tdT-ε2A5. Cells were fixed in 100% methanol and stained with Coomassie blue (n = 3). (C). Transmigration of HT1080tdT and HT1080tdT-ε2A5 across an 8 µm pore membrane. *p < 0.0001, n = 3. The images are representative of more than 3 biological replicates.