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. 2022 Sep 30;10:896297. doi: 10.3389/fcell.2022.896297

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Copyright © 2022 Groenendyk, Stoletov, Paskevicius, Li, Dai, Pujol, Busaan, Ng, Boukouris, Saleme, Haromy, Cui, Hu, Yan, Zhang, Michelakis, Chen, Lewis, Tang, Agellon and Michalak.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HT1080tdT cells extravasation in zebrafish embryos. (A) and (B). Representative fluorescent (left) and confocal (right) images of transgenic zebrafish embryos Tg (fli1:EGFP) injected with HT1080tdT cells (A) or HT1080tdT-ε2A5 cells (B). Fluorescent images were taken 3 days post injection, whereas confocal images were taken 5 days post injection. White arrows indicate the position of migrated HT1080tdT cells. (C). Quantification of migrated HT1080tdT cancer cells at 3 days post injection; HT1080tdT, n = 16; HT1080tdT-ε2A5, n = 24, ***p < 0.0001. The images in the figure are representative of more than 3 biological replicates.