Table 1. Quantitect Primers and Primer Sequences Used for RT-qPCR Analysis.
quantitect primers | mouse | |
---|---|---|
Gapdh | Mm_Gapdh 3_SG QuantiTect Primer Assay—QT01658692 | |
Il1b | Mm_Il1b_2_SG QuantiTect Primer Assay—QT01048355 | |
Il6 | Mm_Il6_1_SG QuantiTect Primer Assay—QT00098875 | |
Tnfa | Mm_Tnf_1_SG QuantiTect Primer Assay—QT00104006 | |
mouse | ||
Gene | 5′-Forward Sequence-3′ | 5′-Reverse Sequence-3′ |
Gapdh | AGGTCGGTGTGAACGGATTTG | TGTAGACCATGTAGTTGAGGTCA |
Il1b | GCAACTGTTCCTGAACTCAACT | ATCTTTTGGGGTCCGTCAACT |
Il6 | TAGTCCTTCCTACCCCAATTTCC | TTGGTCCTTAGCCACTCCTTC |
Tnfa | CCCTCACACTCAGATCATCTTCT | GCTACGACGTGGGCTACAG |
Ces1c | CTCAGATATGTTCAGCACCGAAA | GCTTTTTGTCAAATCGGCAGG |
Ces1d | ATATGGCTTTCTCTTGCTGCG | CCCAGGACTTTGCCTTTAACAGT |
Ces2g | TCTCTGAGGTGGTTTACCAAACG | CCTCTCAGACAGCGCACCAG |
human | ||
Gene | 5′-Forward Sequence-3′ | 5′-Reverse Sequence-3′ |
IL6 | AGCCACTCACCTCTTCAGAAC | GCCTCTTTGCTGCTTTCACAC |
TNFa | CCCATGTTGTAGCAAACCCT | TGAGGTACAGGCCCTCTGAT |
CCL3 | TTCCGTCACCTGCTCAGAAT | CAGCAGCAAGTGATGCAGAGA |
GAPDH | GAPDH_1_SG QuantiTect Primer Assay—QT00079247 |