Figure 1.

Organoselenium compound ebselen is an inhibitor of the YTH domain. (A) Binding specificity of the YTH domain toward m6A compared to adenosine. Data were fitted using a three-parameter nonlinear regression model R² = 0.9870, EC₅₀ = 596 μM. NFUs = normalized fluorescence units. (B) Fluorescence quenching by the ssRNA containing the GGm6ACU variant compared to the unmethylated one. Data were fitted using a three-parameter nonlinear regression model. R² = 0.9386, EC₅₀ = 1.05 μM. NFUs = normalized fluorescence units. (C) Fluorescence quenching by the ssRNA containing the GAm6ACU consensus variant compared to the unmethylated one. Data were fitted using a three-parameter nonlinear regression model. R² = 0.9484, EC₅₀ = 0.139 μM. NFUs = normalized fluorescence units. (D) Plot of progressive Z-score values of fluorescence intensity (FI) of 2560 compounds according to their quenching effect on the YTHDF1 domain. (E) Molecular structure of ebselen (1), the selected hit compound.