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. 2022 Sep 14;5(10):872–891. doi: 10.1021/acsptsci.2c00008

Figure 1.

Figure 1

Organoselenium compound ebselen is an inhibitor of the YTH domain. (A) Binding specificity of the YTH domain toward m6A compared to adenosine. Data were fitted using a three-parameter nonlinear regression model R2 = 0.9870, EC50 = 596 μM. NFUs = normalized fluorescence units. (B) Fluorescence quenching by the ssRNA containing the GGm6ACU variant compared to the unmethylated one. Data were fitted using a three-parameter nonlinear regression model. R2 = 0.9386, EC50 = 1.05 μM. NFUs = normalized fluorescence units. (C) Fluorescence quenching by the ssRNA containing the GAm6ACU consensus variant compared to the unmethylated one. Data were fitted using a three-parameter nonlinear regression model. R2 = 0.9484, EC50 = 0.139 μM. NFUs = normalized fluorescence units. (D) Plot of progressive Z-score values of fluorescence intensity (FI) of 2560 compounds according to their quenching effect on the YTHDF1 domain. (E) Molecular structure of ebselen (1), the selected hit compound.