REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Goat anti-human IgG-HRP	Jackson ImmunoResearch	109-036-098; RRID: AB_2337596
mAb CR3022	Produced for this paper	N/A
mAb CR9114	Produced for this paper	N/A
mAb 1E9F9	Absolute Antibody	Ab01402–2.0
Bacterial and virus strains
SARS-CoV-2 VSV pseudotypes	Generated for this paper	N/A
Biological samples
Acutely SARS-CoV-2 infected HCW serum samples	The University of Pennsylvania	Gouma et al. (2021)
SARS-CoV-2 mRNA vaccinated participants serum samples	The University of Pennsylvania	Goel et al. (2021a)
Chemicals, peptides, and recombinant proteins
SARS-CoV-2 spike protein	Produced for this paper	N/A
SARS-CoV-2 S1 subunit protein	ACROBiosystems	Cat. S1N-C52H3
SARS-CoV-2 S2 subunit protein	ACROBiosystems	Cat. S2N-C52H5
OC43 spike protein	Sino Biological	Cat. 40607-V08B
OC43 S1 subunit protein	Produced for this paper	N/A
OC43 S2 subunit protein	Produced for this paper	N/A
HKU1 spike protein	Sino Biological	Cat. 40606-V08B
HKU1 S1 subunit protein	Sino Biological	Cat. 40021-V08H
HKU1 S2 subunit protein	Sino Biological	Cat. 40021-V08B
SARS-CoV spike protein	Sino Biological	Cat. 40634-V08B
SARS-CoV S1 subunit protein	Sino Biological	Cat. 40150-V08B1
SARS-CoV S2 subunit protein	Sino Biological	Cat. 40150-V08B3
229E spike protein	Sino Biological	Cat. 40605-V08B
IVR-190 H1N1 rHA	Produced for this paper	N/A
Experimental models: Cell lines
293T	ATCC	Cat. CRL-3216, RRID:CVCL_0063
293F	Laboratory of Scott Hensley, University of Pennsylvania, PA	Thermo Fisher cat. R79007
VeroE6/TMPRSS2	Laboratory of Stefan Pohlman, German Primate Center, Leibniz Institute for Primate Research	Hoffmann et al. (2020)
Recombinant DNA
Plasmid: pCAGGS SARS-CoV-2 spike	Laboratory of Florian Krammer, Mt. Sinai, NY	Amanat et al. (2020)
Plasmid: pCG1 SARS-2 S	Laboratory of Stefan Pohlman, German Primate Center, Leibniz Institute for Primate Research	Hoffmann et al. (2020)
Plasmid: OC43 rS1	Laboratory of Scott Hensley, University of Pennsylvania, PA	Anderson et al. (2021)
Plasmid: OC43 rS2	Laboratory of Scott Hensley, University of Pennsylvania, PA	Anderson et al., (2021)
Plasmid: mAb CR3022 HC	Laboratory of Ian Wilson, Scripps Research Institute, CA	Yuan et al. (2020)
Plasmid: mAb CR3022 LC	Laboratory of Ian Wilson, Scripps Research Institute, CA	Yuan et al. (2020)
Plasmid: mAb CR9114 HC	Laboratory of Ian Wilson, Scripps Research Institute, CA	Dreyfus et al. (2012)
Plasmid: mAb CR9114 LC	Laboratory of Ian Wilson, Scripps Research Institute, CA	Dreyfus et al. (2012)
Software and algorithms
Prism8	GraphPad Software	www.graphpad.com/scientific-software/prism/