Fig. 3.

RAD6 inhibition induces multinucleation and giant nuclei formation. MDA-MB-468 (A-C) and HCC1937 (D-F) cells were treated with the indicated doses of paclitaxel (PTX) for 24 h, and 100 viable cells were replated as single cells in quadruplicates post PTX treatment. Colonies were allowed to form and were then either left untreated or treated with 0.75 µM SMI#9. A, D Colony forming efficiency. A **P < 0.005 and one-way ANOVA, P = 0.022; D *P < 0.01, **P < 0.005 and one-way ANOVA, P = 0.0002. B, E Quantitation of colonies containing giant or multinucleated abnormal cells. Results are expressed as mean ± S.E.M. of replicates. E One-way ANOVA, P = 0.038. C, F Representative phase contrast images (long arrow, multinucleated cells; short arrow, cell with giant nucleus). Scale bars, 20 μM