Fig. 7.
SMI#9 reverts paclitaxel (PTX) resistance. A PTX sensitivities of isogenic parental PTX-resistant MDA-MB-468 cells. B Representative phase-contrast micrographs. Original magnification × 20. C Quantification of cells with enlarged or multiple nuclei. Data expressed as mean ± S.E.M. D Immunofluorescence staining of TAU. Bar, 10 µm. E MDA-MB-468 PTXR cells were treated with various doses of PTX with or without 0.75 µM SMI#9 and proliferating cells measured by MTT assays. Data are expressed relative to control cells as mean ± S.E.M. G. Representative phase-contrast images are shown. Cells with multiple or giant nuclei are indicated by arrow. Scale bar, 10 μM. F Combination indices calculated with the CompuSyn software where a CI > 1 indicates antagonism, CI < 1 indicates synergy and CI = 1 indicates an additive effect