Figure 2.

Inhibition of Sa biofilm growth and synthesis of polysaccharide by gurmarin polypeptide. (A) Sa biofilm growth was determined with purified gurmarin (1 µg/ml) in glass test tubes and TSB with gentle shaking (100 rpm) for 16 h at 37°C. After removing the medium and unbound cells, the biofilm was stained with crystal violet (0.1%). (B) Immunoblot analysis of N-acetylglucosamine polysaccharide from control and gurmarin-treated Sa biofilms. Control and gurmarin-treated Sa biofilm cell lysates with equal amounts of protein content were resolved on SDS-PAGE and immunoblotted with anti-PNAG antibody (upper panel). Biofilm proteins separated on SDS-PAGE similarly and stained with Coomassie brilliant blue (CBB) are also shown (lower panel).