FIG 5.

Inhibition of PBP activity by xeruborbactam in bacterial membrane preparations. Incubation of bacterial membrane preparations with control beta-lactams was performed for 10 min at 30°C; xeruborbactam was incubated with membrane preparations for 1 h at 30°C. Bocillin FL fluorescent probe was added, and the reaction mixture was incubated for an additional 10 min at 30°C in the case of beta-lactams or for 20 min on ice (0°C) in the case of xeruborbactam, to prevent displacement of xeruborbactam from its complex by the Bocillin probe.