TABLE 6.
Effects of mutations causing increased xeruborbactam MICs on enhancement of antibiotic potency by xeruborbactam through KPC or NDM inhibition
| Strain | Description | MIC (μg/mL) of: |
||||||
|---|---|---|---|---|---|---|---|---|
| Xeruborbactam | Meropenem |
Cefepime |
||||||
| Alone | With xeruborbactam at: |
Alone | With xeruborbactam at: |
|||||
| 4 μg/mL | 8 μg/mL | 4 μg/mL | 8 μg/mL | |||||
| KPM1271 | KPM1026a/KPC-3a | 16 | 16 | ≤0.06 | ≤0.06 | 4 | ≤0.06 | ≤0.06 |
| KPM4127 | KPM3532 (TolQ::M189K)/KPC-3 | 32 | 32 | 0.125 | ≤0.06 | 8 | ≤0.06 | ≤0.06 |
| KPM4129 | KPM3532 (TolQ::M189K)/NDM-1 | 32 | >64 | 0.25 | 0.125 | 8 | ≤0.06 | ≤0.06 |
| KPM1281 | KPM1026a/NDM-1a | 16 | >64 | 0.125 | ≤0.06 | 16 | ≤0.06 | ≤0.06 |
| KPM4128 | KPM3534 (TolQ::S31F)/KPC-3 | 32 | 32 | 0.125 | ≤0.06 | 32 | 0.25 | 0.125 |
| KPM4130 | KPM3534 (TolQ::S31F)/NDM-1 | 32 | >64 | 0.25 | 0.125 | 32 | 0.25 | 0.125 |
a
KPC-3 and NDM-1 plasmids were introduced into KPM1026a and its mutants by conjugation using K. pneumoniae KP1074 and E. coli EC1061 as the donor, respectively (see Materials and Methods).