FIG 5.

Cell chains are not involved in the propagation of ICEBs1 in a biofilm. (A) WT donor cells were mated with recipient cells lacking different autolysins and incubated on a nonbiofilm (MSNc) medium. (B) WT donor cells were mated with recipient cells overexpressing lytABC under the control of an IPTG-inducible promoter and incubated on a biofilm-inducing (MSgg) medium with 50 μM IPTG. For panels A and B, cells were mixed at a 1:5 donor-to-recipient ratio and incubated for 20 h at 30°C. Statistical analysis was performed using an ANOVA for panel A and t tests for panel B; (ns, P > 0.05) and showed no difference. The results shown are representative of at least 3 independent experiments, and error bars represent the standard deviation (SD).