TABLE 2.
MICs for PAO1 and PA14 induced to express OXA-10, OXA-14, and OXA-935
| MICs (μg/mL) |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Straina | aa differences | ATMb | FEP | CAZ | CZAc | C/T | TZPc | FDC | MEM | IPM | I-Rc |
| PAO1 + pPSV37 | NA | 4 | 1 | 1 | 1 | 0.25 | 2 | <0.03 | 0.5 | <2 | 0.25 |
| PAO1 + pPSV37-oxa10 | 8 | 4 | 1 | 1 | 0.5 | 16 | <0.03 | 1 | <2 | 0.5 | |
| PAO1 + pPSV37-oxa14 | G157D | 8 | 4 | 16 (ns)d | 8 | 2 | 8 | 0.12 | 0.5 | <2 | 0.25 |
| PAO1 + pPSV37-oxa935 | F153S, G157D | 4 | 4 | 32 (ns) | 32 (ns) | 4 | 2 | 0.12 | 0.5 | <2 | 0.25 |
| PA14 + pPSV37 | NA | 4 | 1 | 1 | 2 | 0.5 | 2 | 2 | 0.5 | <2 | 0.5 |
| PA14 + pPSV37-oxa10 | 8 | 4 | 1 | 2 | 0.5 | 32 (ns) | 4 | 0.5 | <2 | 0.5 | |
| PA14 + pPSV37-oxa14 | G157D | 8 | 4 | 16 (ns) | 16 (ns) | 2 | 8 | 8 (ns) | <0.25 | <2 | 0.5 |
| PA14 + pPSV37-oxa935 | F153S, G157D | 8 | 4 | 32 (ns) | 16 (ns) | 2 | 2 | 4 | <0.25 | <2 | 0.5 |
a
All strains were induced with 1 mM IPTG to drive the expression of various OXA proteins.
b
ATM, aztreonam; FEP, cefepime; CAZ, ceftazidime; CZA, ceftazidime-avibactam; C/T, ceftolozane-tazobactam; TZP, piperacillin-tazobactam; MEM, meropenem; IPM, imipenem; I-R, imipenem-relebactam; FDC, cefiderocol.
c
Avibactam, tazobactam and relebactam were present at a fixed dose of 4 μg/mL.
d
(ns), nonsusceptible (intermediate and resistant).