TABLE 1.
Bacterial strains used in this study
| Strain | Description | Source or reference |
|---|---|---|
| E. coli strains | ||
| XL1-Blue | General cloning strain | Stratagene |
| SA30B | DNA methylation strain | 47 |
| S. aureus strains | ||
| MN8 | Prototypic menstrual TSS strain, tst+ | 53 |
| MN8 ΔccpA | MN8 containing a deletion of the ccpA gene | This study |
| MN8 Δagr | MN8 with the agr operon replaced with a tetR marker | 31 |
| MN8 Δpsmα | MN8 containing a deletion in the psmα operon | 17 |
| MN8 ΔsrrAB | MN8 containing deletions of the srrA and srrB genes | 15 |
| MN8 ΔsarA | MN8 containing a deletion of the sarA gene | 40 |
| MN8 Δrot | MN8 containing a deletion of the rot gene | 17 |
| MN8 ΔccpA (+ccpA) | MN8 ΔccpA containing pRMC2::ccpA | This study |
| MN8 ΔccpA (pRMC2) | MN8 ΔccpA containing pRMC2 | This study |
| MN8 (pAmilux::Ptst) | MN8 containing pAmilux::Ptst | 40 |
| MN8 ΔccpA (pAmilux::Ptst) | MN8 ΔccpA containing pAmilux::Ptst | This study |
| MN8 Δagr (pAmilux::Ptst) | MN8 Δagr containing pAmilux::Ptst | 17 |
| MN8 ΔsrrAB (pAmilux::Ptst) | MN8 ΔsrrAB containing pAmilux::Ptst | 15 |
| MN8 ΔsarA (pAmilux::Ptst) | MN8 ΔsarA containing pAmilux::Ptst | 40 |
| MN8 Δrot (pAmilux::Ptst) | MN8 Δrot containing pAmilux::Ptst | 17 |