TABLE 3.
Primers used in this study
| Primer | Sequence (5′–3′) |
|---|---|
| pKOR-ccpA-upstream-Fora | GGGGACAAGTTTGTACAAAAAAGCAGGCTAAAGAAGCTGGCCGTACGAA |
| pKOR-ccpA-upstream-Rev | AACTGTCATAATTTCCTCCTTG |
| pKOR-ccpA-downstream-Forb | ACAAAATAAATTCACAAAATTAGGC |
| pKOR-ccpA-downstream-Reva | GGGGACCACTTTGTACAAGAAAGCTGGGTGCATATGCACCGCCATAGTG |
| CcpA-complement-KpnI-Forc | ACACGGTACCTGAAAACGTTTACAAGGAGGA |
| CcpA-complement-SacI-Revc | CCCCCGAGCTCTTATTTTGTAGTTCCTCGGTA |
a
attB sites used for recognition by BP clonase are shown in boldface.
b
Primer contained a 5′ phosphate group for blunt end ligation.
c
Restriction sites (indicated in the primer name) are underlined in the primer sequence.