Figure 3.

Respiratory effects of SSCI-4 and SSCI-5 in anesthetized rats.

For SSCI-5 (A) and SSCI-4 (B), respiration rate (breaths per minute, bpm) is plotted versus time (minutes, min) for the in vivo rat CVR assay. In both panels, the black traces represent the respiration rate in animals that were only treated with vehicle (DMF) over 60 minutes. The dashed vertical lines represent the start and end of supplemental ventilation for animals in the black and green trace (A) or red and blue traces (B). In A, the blue trace is the respiration rate during infusion with SSCI-5 at 30 mg/kg without ventilation. The green trace is the respiration rate during infusion with SSCI-5 at 30 mg/kg with ventilation. In B, the blue and red traces are the respiration rate in two animals during infusion with SSCI-4 at 10 mg/kg with ventilation. The black trace represents a vehicle (DMF) treated animal. Blood samples were collected for test agent exposures at selected times in the study (see Results). For all traces, infusion of either vehicle or compound was started at 0 minutes; a stable baseline in vehicle was obtained before the start of the infusion and is not represented here.