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Phenotypic and genotypic screening for ESBL producing E. coli isolates using (a) double disc synergy test showing typical keyhole pattern denoting a positive ESBL producing isolate and (b) combination disc test showing increased zone diameter around the discs after addition of clavulanic acid confirming positive ESBL as referred by arrows. (c) Electrophoregram showing amplified amplicon bands at 500 bp denoting positive blaCTX-M gene.
