Fig. 4.

Paracellular BRB permeability is increased in Lama2^−/− retinas. (A-D) P10 WT and Lama2^−/− retinal flat-mounts, injected with biocytin-TMR, stained for lectin. (E,F) Biocytin-TMR M.F.I. in the liver parenchyma (E) and the ratio of biocytin-TMR fluorescence intensities in the retina versus liver parenchyma (F) (n=3 mice/genotype). (G-J) P10 WT and Lama2^−/− retinal flat-mounts stained for occludin and lectin. (K) Occludin coverage of blood vessels (n=3 mice/genotype). (L) Schematic of paracellular biocytin-TMR extravasation in WT, Apcdd1^−/− and Lama2^−/− retinas. Data are mean±s.e.m., analyzed with two-tailed unpaired Student's t-test: NS, not significant, **P<0.02.